Principal Investigator: Zhou Mingguo 

Funding Received: 3.18 Million RMB

Sponsored by: National Natural Science Foundation of China

Project Period: January, 2018─December, 2022

Brief Introduction:

  The interaction between fungicide active molecules and receptor/action targets and the regulatory mechanism of pathogens against fungicide stress are theoretical basis of creating highly active, lowly toxic and selective fungicides, and of studying fungicide pharmacology and its efficient and reduced application technology. With Fusarium asiaticum causing wheat scab disease and general microtubulin inhibitor carbendazim as materials, with two important fungicide molecular targets of β1-tubulin and β2-tubulin as objects, this project attempts to study and reveal the molecular specific interaction between fungicides and two molecular targets using a modern molecular biology technology, providing some theories for specially creating highly active and selective microtubulin inhibitors based on microtubulins of different pests; to study the interaction between fungicide active molecules and microtubulin from natural variation and to reveal the genetic mechanism of pathogen with sensitivity and resistance variation, providing some theories for creating highly active and anti-resistant fungicides; to study the regulatory mechanism of common fungicide-resistance mutation genotypes of microtubulin on fitness key properties such as drug-sensitivity, mycelial growth, reproduction and virulence, and to reveal the genetic mechanism of drug-sensitivity difference in pathogen populations, providing the theoretic basis for highly efficient utilization of fungicides and development of new and subversive resistance management technologies.