Principal Investigator: Chen Fadi
Funding Received: 3.14 Million RMB
Sponsored by: National Natural Science Foundation of China
Project Period: January, 2018─December, 2022
Brief Introduction:
Chrysanthemum, one of the top ten traditional famous flowers in China and one of the four cut flowers in the world, is of great ornamental and economic values. Chrysanthemum is readily sensitive to waterlogging which greatly hampers its growth, yield and quality. We have found that the expression levels of CmACS1/6/7 encoding ethylene and its biosynthesis rate limit enzyme ACC synthases (ACS) and CmMBF1c, as the homolog of multiprotein bridging factor 1c (MBF1c) is both induced by waterlogging, and is higher in waterlogging tolerant chrysanthemum cultivars than that in the sensitive ones. The promoter of CmMBF1c is more sensitive to hypoxia by transient assay, and CmMBF1c may interact with the ET signaling member of CmHRE2. We suppose that CmACS1/6/8 and CmMBF1c may be involved in the ET signaling waterlogging tolerance in chrysanthemum. However, their mechanisms remain unknown. Here, we attempt to clone CmACS1/6/8 and CmMBF1c, and perform functional analysis of both the genes, and aim to discover those transcription factors regulating CmACS genes transcription with yeast one-hybrid and the key regulatory and interacting genes of CmMBF1c involved in ethylene signaling to waterlogging, to identify the key genes’ functions and regulatory traits via generating corresponding RNA interfering and overexpression transformants, and mining the downstream differential gene expression profiles in the transformants by transcriptome sequencing, and to disclose the regulatory roles of regulatory and interacting genes in regulating pathway by using EMSA, BiFC, CoIP and ChIP. To sum up, the present proposal will discover a number of key genes involved in ethylene mediated waterlogging response and the molecular mechanisms underlying waterlogging tolerance, which in turn will give aid to genetic manipulation of waterlogging tolerance in chrysanthemum by providing candidate genes and theoretic guidance.
